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We are very proud of our history and the core values that have contributed to our success in the field of laboratory diagnosis. Microbiology at Dhulikhel Hospital/Kathmandu University Hospital (DH-KUH) is an appropriate and reliable place for proper diseases diagnosis. As a department, our major concern is the provision of excellent laboratory diagnosis services and effective academic environment. We also have discipline, dedication and determination - something that is entirely necessary to the development of the scientific mind.


Our mission is to provide excellent service in the diagnostic arena, along with highest quality of research and education possible in the interdisciplinary fields of Microbiology and immunology in the perform research of international standard and provide education for the production of health manpower with quality and morality.


A. Laboratory Diagnosis Service:-

  • Bacteriology and mycology:- Identification of clinical isolates via microscopy and biochemical tests, their confirmation and antibiotic sensitivity pattern are performed. Blood culture, urine culture and culture of various body fluids has been done routinely. Isolation of bacteria and fungus has certainly helped in the diagnosis of clinical conditions. Molecular identification of tuberculosis has been in service since 2012.
  • Parasitology:- Routine and microscopic examination of stool and urine are performed regularly, whereas special tests are performed whenever necessary.
  • Virology:- Various viral diseases are diagnosed by serological investigations so far. We have recently started molecular detection of Human Papilloma Virus (HPV).
  • Immunology:- The establishment of the immunology unit is a recent advancement in the department. Almost 40 parameters are included in order to strengthen the diagnostic fraternity of our hospital that includes TORCH profile, Emergency Immunology, Hepatitis Profile and Immunology of infectious diseases.
  • Molecular Laboratory:- The establishment of molecular laboratory in 2011 has certainly added the range of diagnosis. The department also enjoys the facilities such as diagnostic tools like PCR for the testing of Mycobacteria, and HPV detection are already in service. Beside this, molecular laboratory has been in action for various national and international research projects.

Quality control (QC) is of utmost priority for us. Since September 2007 external quality control is being addressed in association with National Public Health Laboratory (NPHL) and Nepal Tuberculosis Center (NTC). Internal quality control is done regularly by performing culture, biochemical tests and microscopy etc.

 B. Medical Education and Research Activities:-

We have been a part of MBBS, BDS, BPT, B.Sc Nursing and BNS program providing knowledge to students on different aspects of microbiology. We have a strong collaboration with international universities like Mahidol University (MU, Thailand), Norwegian Institute of Science and Technology (NTNU, Norway). Visiting faculties come to our Hospital to update us with the global scenario of microbiology.

Research is a cornerstone of our academic effort and our research programs in Microbiology and Immunology are increasing. With steadily increasing research values, our work is increasingly breaking new ground.

Microbiology Tests List:

Future Plan:

  • Installation of Flow cytometer
  • Establishment of Molecular diagnosis settings
  • Virology laboratory
  • HIV research collaborations
  • Mycobacterial ,Anaerobic and fungal culture
  • Improve the research infrastructure by expanding the core facilities, with support from the administration and made possible by a doubling of the grant funding coming into the department.